Catalogue Number: ELK4404
Alternative Names: KIM-3; TIM3; TIMD3; Tim-3; T-cell membrane protein 3; T-cell immunoglobulin mucin receptor 3; T-cell immunoglobulin and mucin domain-containing protein 3
Species: Human
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research: Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatitis A Virus Cellular Receptor 2(HAVCR2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatitis A Virus Cellular Receptor 2(HAVCR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatitis A Virus Cellular Receptor 2(HAVCR2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatitis A Virus Cellular Receptor 2(HAVCR2) in the samples is then determined by comparing the OD of the samples to the standard curve.