{"product_id":"human-hbf-fetal-hemoglobin-elisa-kit-elk10343","title":"Human HBF (Fetal Hemoglobin) ELISA Kit - ELK10343","description":"\u003cp\u003eHuman HBF (Fetal Hemoglobin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10343-48, ELK10343-96, ELK10343-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: hemoglobin F; Foetal Haemoglobin\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.99 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 µg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, erythrocyte lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Hematology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBF in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200660988083,"sku":"ELK10343-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200661020851,"sku":"ELK10343-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200661053619,"sku":"ELK10343-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_c19ab1a9-cbee-4bcc-b108-cfc35f64cedd.png?v=1767061184","url":"https:\/\/afsbio.com\/products\/human-hbf-fetal-hemoglobin-elisa-kit-elk10343","provider":"AFSBio Inc.","version":"1.0","type":"link"}