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Human HGFAC (Hepatocyte Growth Factor Activator) ELISA Kit

Human HGFAC (Hepatocyte Growth Factor Activator) ELISA Kit

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Catalogue Number: ELK4505

Alternative Names: HGFA; HGFAP; HGF Activator

Species: Human

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Immune molecule;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor Activator(HGFAC). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Growth Factor Activator(HGFAC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Growth Factor Activator(HGFAC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Growth Factor Activator(HGFAC) in the samples is then determined by comparing the OD of the samples to the standard curve.