{"product_id":"human-hla-b27-leukocyte-antigen-b27-elisa-kit-elk0864","title":"Human HLA-B27 (Leukocyte Antigen B27) ELISA Kit - ELK0864","description":"\u003cp\u003eHuman HLA-B27 (Leukocyte Antigen B27) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0864-48, ELK0864-96, ELK0864-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: HALB27; Human leukocyte antigen B27\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.12 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Immune molecule\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-B27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-B27 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: O19193\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212927434931,"sku":"ELK0864-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212927467699,"sku":"ELK0864-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200868966579,"sku":"ELK0864-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_b54a97d3-1a12-4131-a02f-254266cb7b41.png?v=1767066120","url":"https:\/\/afsbio.com\/products\/human-hla-b27-leukocyte-antigen-b27-elisa-kit-elk0864","provider":"AFSBio Inc.","version":"1.0","type":"link"}