Catalogue Number: ELK4646
Alternative Names: FJHN; TCF2; HNF1βeta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2,Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta(HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta(HNF1b) in the samples is then determined by comparing the OD of the samples to the standard curve.