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Human HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit

Human HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit

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Catalogue Number: ELK4646

Alternative Names: FJHN; TCF2; HNF1βeta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2,Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Cytokine;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta(HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta(HNF1b) in the samples is then determined by comparing the OD of the samples to the standard curve.