Catalogue Number: ELK4601
Alternative Names: 2-O-sulfotransferase; 2OST; dJ604K5.2; FLJ11317; heparan sulfate 2 O sulfotransferase 1; Heparan sulfate 2-O-sulfotransferase 1; HS2ST_HUMAN; hs2st1; KIAA0448; MGC131986
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1(HS2ST1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparan Sulfate-2-O-Sulfotransferase 1(HS2ST1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-2-O-Sulfotransferase 1(HS2ST1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-2-O-Sulfotransferase 1(HS2ST1) in the samples is then determined by comparing the OD of the samples to the standard curve.