Catalogue Number: ELK4598
Alternative Names: HS6ST3; Heparan-sulfate 6-O-sulfotransferase 3; HS6ST-3;
Species: Human
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3) in the samples is then determined by comparing the OD of the samples to the standard curve.