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Human HS6ST3 (Heparan Sulfate-6-O-Sulfotransferase 3) ELISA Kit

Human HS6ST3 (Heparan Sulfate-6-O-Sulfotransferase 3) ELISA Kit

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Catalogue Number: ELK4598

Alternative Names: HS6ST3; Heparan-sulfate 6-O-sulfotransferase 3; HS6ST-3;

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Enzyme & Kinase;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 3(HS6ST3) in the samples is then determined by comparing the OD of the samples to the standard curve.