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Human HSP90aB1 (Heat Shock Protein 90kDa Alpha B1) ELISA Kit

Human HSP90aB1 (Heat Shock Protein 90kDa Alpha B1) ELISA Kit

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Catalogue Number: ELK4467

Alternative Names: HSP90-BETA; HSP90B; HSPC2; HSPCB; HSP84; Heat Shock 90kD Protein 1,Beta; Heat shock 84 kDa

Species: Human

Assay Type: Sandwich

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Alpha B1(HSP90aB1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Alpha B1(HSP90aB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Alpha B1(HSP90aB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Alpha B1(HSP90aB1) in the samples is then determined by comparing the OD of the samples to the standard curve.