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Human HSPF2 (Heat Shock 40kDa Protein 2) ELISA Kit

Human HSPF2 (Heat Shock 40kDa Protein 2) ELISA Kit

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Catalogue Number: ELK4519

Alternative Names: DNAJC4; MCG18; HSP-F2; DnaJ(Hsp40)Homolog,Subfamily C,Member 4; DnaJ-like protein HSPF2; Multiple endocrine neoplasia type 1 candidate protein number 18

Species: Human

Assay Type: Sandwich

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 40kDa Protein 2(HSPF2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 40kDa Protein 2(HSPF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 40kDa Protein 2(HSPF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 40kDa Protein 2(HSPF2) in the samples is then determined by comparing the OD of the samples to the standard curve.