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Human IL11Ra (Interleukin 11 Receptor Alpha) ELISA Kit

Human IL11Ra (Interleukin 11 Receptor Alpha) ELISA Kit

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Catalogue Number: ELK4402

Alternative Names: CRSDA; hCG_2011440; IL-11 receptor subunit alpha; IL-11R subunit alpha; IL-11R-alpha; IL-11RA; IL11RA; Interleukin 11 receptor alpha ; Interleukin 11 receptor alpha chain; Interleukin-11 receptor subunit alpha; MGC2146

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 11 Receptor Alpha(IL11Ra). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 11 Receptor Alpha(IL11Ra). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 11 Receptor Alpha(IL11Ra), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 11 Receptor Alpha(IL11Ra) in the samples is then determined by comparing the OD of the samples to the standard curve.