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Human IL2Rg (Interleukin 2 Receptor Gamma) ELISA Kit

Human IL2Rg (Interleukin 2 Receptor Gamma) ELISA Kit

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Catalogue Number: ELK4699

Alternative Names: CD132; IL2R-g; IL2-RG; CD132; IMD4; p64; SCIDX; SCIDX1; Cytokine Receptor Common Gamma Chain; Severe Combined Immunodeficiency

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Length: 3.5h

Research: Signal transduction;Infection immunity;Immunodeficiency;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 2 Receptor Gamma(IL2Rg). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 2 Receptor Gamma(IL2Rg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 2 Receptor Gamma(IL2Rg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 2 Receptor Gamma(IL2Rg) in the samples is then determined by comparing the OD of the samples to the standard curve.