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Human ISL1 (ISL LIM Homeobox Protein 1) ELISA Kit

Human ISL1 (ISL LIM Homeobox Protein 1) ELISA Kit

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Catalogue Number: ELK4406

Alternative Names: Islet-1; ISL1 Transcription Factor,LIM/Homeodomain; Insulin gene enhancer protein ISL-1

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ISL LIM Homeobox Protein 1(ISL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ISL LIM Homeobox Protein 1(ISL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ISL LIM Homeobox Protein 1(ISL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ISL LIM Homeobox Protein 1(ISL1) in the samples is then determined by comparing the OD of the samples to the standard curve.