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Human ITGa2B (Integrin Alpha 2B) ELISA Kit

Human ITGa2B (Integrin Alpha 2B) ELISA Kit

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Catalogue Number: ELK4630

Alternative Names: CD41; CD41B; ITG-A2B; GTA; GP2B; GPIIb; HPA3; Platelet Glycoprotein IIb Of IIb/IIIa Complex; Antigen CD41

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: CD & Adhesion molecule;Tumor immunity;Infection immunity;Hematology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Integrin Alpha 2B(ITGa2B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Integrin Alpha 2B(ITGa2B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Integrin Alpha 2B(ITGa2B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Integrin Alpha 2B(ITGa2B) in the samples is then determined by comparing the OD of the samples to the standard curve.