Catalogue Number: ELK4527
Alternative Names: HLA-A; HLAA; MHC-A; Leukocyte Antigen A; HLA class I histocompatibility antigen, A-31 alpha chain
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Immune molecule;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I A(MHCA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I A(MHCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I A(MHCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I A(MHCA) in the samples is then determined by comparing the OD of the samples to the standard curve.