{"product_id":"human-mhce-major-histocompatibility-complex-class-i-e-microsample-elisa-kit-elk10370ms","title":"Human MHCE (Major Histocompatibility Complex Class I E) Microsample ELISA Kit - ELK10370MS","description":"\u003cp\u003eHuman MHCE (Major Histocompatibility Complex Class I E) Microsample ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK10370MS-48, ELK10370MS-96, ELK10370MS-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: COL17A1; BP180; BPAG2; LAD-1; LABD97; Collagen Alpha-1 (XVII)chain; 180 kDa bullous pemphigoid antigen 2; Bullous pemphigoid antigen 2; Linear IgA disease antigen 1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.1 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway; Developmental science; Nutrition metabolism; Dermatology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL17. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL17. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL17 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45199925412019,"sku":"ELK10370MS-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45199925444787,"sku":"ELK10370MS-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45199925477555,"sku":"ELK10370MS-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_d554778d-48d0-43e1-b1b7-247f3e1b70ae.png?v=1767057085","url":"https:\/\/afsbio.com\/products\/human-mhce-major-histocompatibility-complex-class-i-e-microsample-elisa-kit-elk10370ms","provider":"AFSBio Inc.","version":"1.0","type":"link"}