{"product_id":"human-n-mid-ot-n-midosteocalcin-elisa-kit-elk9587","title":"Human N-MID-OT (N-MIDOsteocalcin) ELISA Kit - ELK9587","description":"\u003cp\u003eHuman N-MID-OT (N-MIDOsteocalcin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9587-48, ELK9587-96, ELK9587-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: N-MID-OT\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.39 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.63-40 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N-MID-OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human N-MID-OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human N-MID-OT in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212830212275,"sku":"ELK9587-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212830245043,"sku":"ELK9587-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200827941043,"sku":"ELK9587-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_1cd11f0a-79bd-4245-b00a-2e79c412fb85.png?v=1767065039","url":"https:\/\/afsbio.com\/products\/human-n-mid-ot-n-midosteocalcin-elisa-kit-elk9587","provider":"AFSBio Inc.","version":"1.0","type":"link"}