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Human NDUFS1 (NADH Dehydrogenase Ubiquinone Fe-S Protein 1) ELISA Kit

Human NDUFS1 (NADH Dehydrogenase Ubiquinone Fe-S Protein 1) ELISA Kit

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Catalogue Number: ELK4661

Alternative Names: CI-75Kd; NADH-Coenzyme Q Reductase; NADH Dehydrogenase Iron-Sulfur Protein 1; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Enzyme & Kinase;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1(NDUFS1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to NADH Dehydrogenase Ubiquinone Fe-S Protein 1(NDUFS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NADH Dehydrogenase Ubiquinone Fe-S Protein 1(NDUFS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NADH Dehydrogenase Ubiquinone Fe-S Protein 1(NDUFS1) in the samples is then determined by comparing the OD of the samples to the standard curve.