{"product_id":"human-nes-nestin-elisa-kit-elk3509","title":"Human NES (Nestin) ELISA Kit - ELK3509","description":"\u003cp\u003eHuman NES (Nestin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK3509-48, ELK3509-96, ELK3509-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: ESTM 46 ; FLJ 21841 ; FLJ21841 ; Intermediate filament protein ; Nbla00170 ; nes ; NEST\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.056 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NES in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P48681\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213383303347,"sku":"ELK3509-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213383336115,"sku":"ELK3509-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200139354291,"sku":"ELK3509-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_84411973-b68d-4a61-b86c-d3d537a4b07b.png?v=1767033657","url":"https:\/\/afsbio.com\/products\/human-nes-nestin-elisa-kit-elk3509","provider":"AFSBio Inc.","version":"1.0","type":"link"}