{"product_id":"human-nfκb-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk1692","title":"Human NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit - ELK1692","description":"\u003cp\u003eHuman NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK1692-48, ELK1692-96, ELK1692-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NFKB3; p65; REL-A; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 3; Transcription Factor P65; Nuclear factor NF-kappa-B p65 subunit; RELA; V-Rel Reticuloendotheliosis Viral Oncogene Homolog A\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.054 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Apoptosis;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q04206\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213077840051,"sku":"ELK1692-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213077872819,"sku":"ELK1692-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200026435763,"sku":"ELK1692-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_29ee33d1-2e3d-40f6-a37a-1a043e4e4322.png?v=1767031056","url":"https:\/\/afsbio.com\/products\/human-nf%ce%bab-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk1692","provider":"AFSBio Inc.","version":"1.0","type":"link"}