{"product_id":"human-nfκb-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk9589","title":"Human NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit - ELK9589","description":"\u003cp\u003eHuman NFκB-p65 (Nuclear Factor Kappa B p65) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9589-48, ELK9589-96, ELK9589-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NFKB3; p65; RelA\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.1 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: Q04206\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213984530611,"sku":"ELK9589-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213984563379,"sku":"ELK9589-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45201430184115,"sku":"ELK9589-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_9edec51a-7605-4ad4-a6c7-d05e224b8475.png?v=1767097183","url":"https:\/\/afsbio.com\/products\/human-nf%ce%bab-p65-nuclear-factor-kappa-b-p65-elisa-kit-elk9589","provider":"AFSBio Inc.","version":"1.0","type":"link"}