{"product_id":"human-nf-κbp105-nuclear-factor-nf-kappa-b-p105-subunit-elisa-kit-elk2228","title":"Human NF-κBp105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit - ELK2228","description":"\u003cp\u003eHuman NF-κBp105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK2228-48, ELK2228-96, ELK2228-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit; NFkB; Nuclear Factor Kappa B\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.056 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NF-κBp105. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NF-κBp105. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NF-κBp105, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P19838\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43213214941363,"sku":"ELK2228-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43213214974131,"sku":"ELK2228-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200049668275,"sku":"ELK2228-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_bd3cd25b-6cb8-4cf6-ac77-e08c112e5c6d.png?v=1767031747","url":"https:\/\/afsbio.com\/products\/human-nf-%ce%babp105-nuclear-factor-nf-kappa-b-p105-subunit-elisa-kit-elk2228","provider":"AFSBio Inc.","version":"1.0","type":"link"}