Catalogue Number: ELK4713
Alternative Names: NFI-RED; NFIB2; NFIB3; CCAAT-box-binding transcription factor; TGGCA-binding protein
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B(NFIB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Factor I/B(NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B(NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor I/B(NFIB) in the samples is then determined by comparing the OD of the samples to the standard curve.