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Human NFIB (Nuclear Factor I/B) ELISA Kit

Human NFIB (Nuclear Factor I/B) ELISA Kit

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Catalogue Number: ELK4713

Alternative Names: NFI-RED; NFIB2; NFIB3; CCAAT-box-binding transcription factor; TGGCA-binding protein

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/B(NFIB). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nuclear Factor I/B(NFIB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/B(NFIB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor I/B(NFIB) in the samples is then determined by comparing the OD of the samples to the standard curve.