{"product_id":"human-nkx2-5-nk2-homeobox-5-elisa-kit-elk0416","title":"Human NKX2-5 (NK2 Homeobox 5) ELISA Kit - ELK0416","description":"\u003cp\u003eHuman NKX2-5 (NK2 Homeobox 5) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0416-48, ELK0416-96, ELK0416-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E; CSX; NKX2.5; NKX2E\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 21.4 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 78.13-5000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX2-5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX2-5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX2-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX2-5 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P52952\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212881952947,"sku":"ELK0416-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212881985715,"sku":"ELK0416-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200861855923,"sku":"ELK0416-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_9cb7b32c-d980-48c0-8cc9-3148365122c6.png?v=1767065791","url":"https:\/\/afsbio.com\/products\/human-nkx2-5-nk2-homeobox-5-elisa-kit-elk0416","provider":"AFSBio Inc.","version":"1.0","type":"link"}