Catalogue Number: ELK4727
Alternative Names: N-CNP
Species: Human
Assay Type: Competitive Inhibition
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research: Cardiovascular biology;Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with N-Terminal Pro-C-Type Natriuretic Peptide(NT-ProCNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-C-Type Natriuretic Peptide(NT-ProCNP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-C-Type Natriuretic Peptide(NT-ProCNP) in the samples is then determined by comparing the OD of the samples to the standard curve.