{"product_id":"human-ofq-n-orphaninfq-nociceptin-elisa-kit-elk4683","title":"Human OFQ\/N (OrphaninFQ\/Nociceptin) ELISA Kit - ELK4683","description":"\u003cp\u003eHuman OFQ\/N (OrphaninFQ\/Nociceptin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK4683-48, ELK4683-96, ELK4683-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: PPNOC; Prepronociceptin; Pocistatin; Pro-nociceptin; Preproorphanin FQ; Neuropeptide 1; Nociceptin; Orphanin FQ; PNOC; Pronociceptin\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.056 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Neuroscience\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human OFQ\/N. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OFQ\/N. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human OFQ\/N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OFQ\/N in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211717509299,"sku":"ELK4683-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211717542067,"sku":"ELK4683-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200729211059,"sku":"ELK4683-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_78e6b5f7-75ee-4869-9dbc-e5c73ed29ab8.png?v=1767062736","url":"https:\/\/afsbio.com\/products\/human-ofq-n-orphaninfq-nociceptin-elisa-kit-elk4683","provider":"AFSBio Inc.","version":"1.0","type":"link"}