{"product_id":"human-p-ampk-phosphorylated-adenosine-monophosphate-activated-protein-kinase-elisa-kit-elk11308","title":"Human p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit - ELK11308","description":"\u003cp\u003eHuman p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11308-48, ELK11308-96, ELK11308-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.121 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.32-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200705552563,"sku":"ELK11308-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200705585331,"sku":"ELK11308-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200705618099,"sku":"ELK11308-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_e1979e6a-aa2a-4c7a-9396-cdacbab616e7.png?v=1767061915","url":"https:\/\/afsbio.com\/products\/human-p-ampk-phosphorylated-adenosine-monophosphate-activated-protein-kinase-elisa-kit-elk11308","provider":"AFSBio Inc.","version":"1.0","type":"link"}