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Human PGLYRP2 (Peptidoglycan Recognition Protein 2) ELISA Kit

Human PGLYRP2 (Peptidoglycan Recognition Protein 2) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4472

Alternative Names: HMFT0141; PGLYRPL; PGRP-L; PGRPL; TAGL-like; tagL; tagL-alpha; tagl-beta; N-acetylmuramoyl-L-alanine amidase; Peptidoglycan recognition protein long

Species: Human

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, urine, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Tumor immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidoglycan Recognition Protein 2(PGLYRP2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peptidoglycan Recognition Protein 2(PGLYRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidoglycan Recognition Protein 2(PGLYRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidoglycan Recognition Protein 2(PGLYRP2) in the samples is then determined by comparing the OD of the samples to the standard curve.