{"product_id":"human-phospho-akt-s473-p-akt-elisa-kit-elk0792","title":"Human Phospho AKT S473 (p-AKT) ELISA Kit - ELK0792","description":"\u003cp\u003eHuman Phospho AKT S473 (p-AKT) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK0792-48, ELK0792-96, ELK0792-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: p-AKT\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 52.5 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 156.25-10000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: tissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Enzyme and Kinase\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43211536171187,"sku":"ELK0792-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43211536203955,"sku":"ELK0792-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200643621043,"sku":"ELK0792-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_2deb74fc-0bec-47c5-8b7a-7d53f2d2e8a3.png?v=1767060785","url":"https:\/\/afsbio.com\/products\/human-phospho-akt-s473-p-akt-elisa-kit-elk0792","provider":"AFSBio Inc.","version":"1.0","type":"link"}