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Human PICALM (Phosphatidylinositol Binding Clathrin Assembly Protein) ELISA Kit

Human PICALM (Phosphatidylinositol Binding Clathrin Assembly Protein) ELISA Kit

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Catalogue Number: ELK4443

Alternative Names: LAP; CALM; CLTH; Clathrin assembly lymphoid myeloid leukemia protein

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol Binding Clathrin Assembly Protein(PICALM). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylinositol Binding Clathrin Assembly Protein(PICALM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylinositol Binding Clathrin Assembly Protein(PICALM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol Binding Clathrin Assembly Protein(PICALM) in the samples is then determined by comparing the OD of the samples to the standard curve.