Catalogue Number: ELK4693
Alternative Names: PAK1; DBK; PRK1; PRKCL1; Protein Kinase C-Like 1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Serine-threonine protein kinase N
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Enzyme & Kinase;Endocrinology;Bone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase N1(PKN1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase N1(PKN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase N1(PKN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase N1(PKN1) in the samples is then determined by comparing the OD of the samples to the standard curve.