Catalogue Number: ELK4708
Alternative Names: NYD-SP27; PLCzeta; Testis-development protein NYD-SP27; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase zeta-1
Assay Type: Sandwich
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Metabolic pathway;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase C Zeta 1(PLCz1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phospholipase C Zeta 1(PLCz1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase C Zeta 1(PLCz1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase C Zeta 1(PLCz1) in the samples is then determined by comparing the OD of the samples to the standard curve.