Catalogue Number: ELK4471
Alternative Names: DSS; CMT1A; CMT1E; GAS3; HMSNIA; HNPP; Sp110; Growth arrest-specific protein 3
Species: Human
Assay Type: Sandwich
Standard: 100 ng/mL
Detection Range: 1.57-100 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Neuro science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peripheral Myelin Protein 22(PMP22). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peripheral Myelin Protein 22(PMP22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peripheral Myelin Protein 22(PMP22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peripheral Myelin Protein 22(PMP22) in the samples is then determined by comparing the OD of the samples to the standard curve.