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Human pNF-H  (Phosphorylated Neurofilament H) ELISA Kit

Human pNF-H (Phosphorylated Neurofilament H) ELISA Kit

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Catalogue Number: ELK4734

Alternative Names: NFH; 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; Neurofilament, Heavy Polypeptide

Species: Human

Assay Type: Sandwich

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphorylated Neurofilament H(pNF-H ). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphorylated Neurofilament H(pNF-H ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphorylated Neurofilament H(pNF-H ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphorylated Neurofilament H(pNF-H ) in the samples is then determined by comparing the OD of the samples to the standard curve.