Catalogue Number: ELK4739
Alternative Names: CT94.2; Cancer/Testis Antigen Family 94,Member 2; Sperm histone P2; Sperm protamine P2
Assay Type: Sandwich
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: Tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research: Reproductive science;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protamine 2(PRM2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protamine 2(PRM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protamine 2(PRM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protamine 2(PRM2) in the samples is then determined by comparing the OD of the samples to the standard curve.