{"product_id":"human-pro-interleukin-1-beta-pro-il1b-elisa-kit-elk11296","title":"Human pro interleukin 1 beta (pro-IL1b) ELISA Kit - ELK11296","description":"\u003cp\u003eHuman pro interleukin 1 beta (pro-IL1b) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11296-48, ELK11296-96, ELK11296-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: pro-IL1B; pro-IL1-Beta; pro-IL1F2; pro-IL-1β\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 11.2 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 31.25-2000 pg\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Cytokine; Tumor immunity; Infection immunity;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pro-IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pro-IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pro-IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pro-IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200704962739,"sku":"ELK11296-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200704995507,"sku":"ELK11296-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200705028275,"sku":"ELK11296-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_928e041c-4d46-4da4-9c59-ef09a9865e33.png?v=1767061904","url":"https:\/\/afsbio.com\/products\/human-pro-interleukin-1-beta-pro-il1b-elisa-kit-elk11296","provider":"AFSBio Inc.","version":"1.0","type":"link"}