Catalogue Number: ELK4434
Alternative Names: CAP; HACD1; Proline Instead Of Catalytic Arginine Member a; 3-hydroxyacyl-CoA dehydratase 1; Cementum Attachment Protein; Very-long-chain 3R-3-hydroxyacyl-CoA dehydratase 1
Species: Human
Assay Type: Sandwich
Standard: 50 ng/mL
Detection Range: 0.79-50 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Tyrosine Phosphatase Like Protein A(PTPLA). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Tyrosine Phosphatase Like Protein A(PTPLA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Tyrosine Phosphatase Like Protein A(PTPLA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Tyrosine Phosphatase Like Protein A(PTPLA) in the samples is then determined by comparing the OD of the samples to the standard curve.