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Human RPA1 (Replication Protein A1) ELISA Kit

Human RPA1 (Replication Protein A1) ELISA Kit

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Catalogue Number: ELK4413

Alternative Names: HSSB; REPA1; RF-A; RP-A; RPA70; Replication protein A 70 kDa DNA-binding subunit; Single-stranded DNA-binding protein

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Signal transduction;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Replication Protein A1(RPA1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Replication Protein A1(RPA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Replication Protein A1(RPA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Replication Protein A1(RPA1) in the samples is then determined by comparing the OD of the samples to the standard curve.