For pricing in CAD please contact us

Human S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit

Human S1PR1 (Sphingosine 1 Phosphate Receptor 1) ELISA Kit

Regular price
$458.00 USD
Regular price
Sale price
$458.00 USD
Unit price
per 
Availability
Sold out

Catalogue Number: ELK4466

Alternative Names: CD363; EDG1; S1-PR1; S1P1; ECGF1; CHEDG1; Endothelial differentiation G-protein coupled receptor 1; Sphingolipid G-Protein-Coupled Receptor 1

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Metabolic pathway;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 1(S1PR1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 1(S1PR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 1(S1PR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 1(S1PR1) in the samples is then determined by comparing the OD of the samples to the standard curve.