{"product_id":"human-scf-stem-cell-factor-microsample-elisa-kit-elk1180ms","title":"Human SCF (Stem Cell Factor) Microsample ELISA Kit - ELK1180MS","description":"\u003cp\u003eHuman SCF (Stem Cell Factor) Microsample ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK1180MS-48, ELK1180MS-96, ELK1180MS-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eReactivity: Human\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Proton pump beta chain; Gastric H (+)\/K (+) ATPase subunit beta\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.05 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.16-10 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATP4b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATP4b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATP4b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATP4b in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eUniProt ID: P51164\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200008675507,"sku":"ELK1180MS-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200008708275,"sku":"ELK1180MS-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200008741043,"sku":"ELK1180MS-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_bb5fdaac-ba61-4368-80e6-ef9cd8f638ed.png?v=1767057381","url":"https:\/\/afsbio.com\/products\/human-scf-stem-cell-factor-microsample-elisa-kit-elk1180ms","provider":"AFSBio Inc.","version":"1.0","type":"link"}