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Human SHP (Small Heterodimer Partner) ELISA Kit

Human SHP (Small Heterodimer Partner) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4427

Alternative Names: NR0B2; SHP1; Nuclear Receptor Subfamily 0,Group B,Member 2; Orphan nuclear receptor SHP

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Signal transduction;Endocrinology;Developmental science;Hormone metabolism;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Small Heterodimer Partner(SHP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Small Heterodimer Partner(SHP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Small Heterodimer Partner(SHP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Small Heterodimer Partner(SHP) in the samples is then determined by comparing the OD of the samples to the standard curve.