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Human TAZ (Tafazzin) ELISA Kit

Human TAZ (Tafazzin) ELISA Kit

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$458.00 USD
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Catalogue Number: ELK4521

Alternative Names: CMD3A; EFE2; EFE; BTHS; XAP-2; G4.5; Barth syndrome; Endocardial Fibroelastosis 2; Cardiomyopathy,Dilated 3A(X-Linked)

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research: Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tafazzin(TAZ). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tafazzin(TAZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tafazzin(TAZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tafazzin(TAZ) in the samples is then determined by comparing the OD of the samples to the standard curve.