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Human Tb4 (Thymosin Beta 4) ELISA Kit

Human Tb4 (Thymosin Beta 4) ELISA Kit

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Catalogue Number: ELK4391

Alternative Names: TMSB4X; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide

Species: Human

Assay Type: Competitive Inhibition

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, plasma and other biological fluids

Assay Length: 2h

Research: Metabolic pathway;Endocrinology;Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Thymosin Beta 4(Tb4) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thymosin Beta 4(Tb4). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymosin Beta 4(Tb4) in the samples is then determined by comparing the OD of the samples to the standard curve.