{"product_id":"human-tpo-ab-anti-thyroid-peroxidase-antibody-elisa-kit-elk9058","title":"Human TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit - ELK9058","description":"\u003cp\u003eHuman TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK9058-48, ELK9058-96, ELK9058-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: TPO Ab; TPO-Ab\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.14 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 3.13-200 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPO-Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":43212757074099,"sku":"ELK9058-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43212757106867,"sku":"ELK9058-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200812736691,"sku":"ELK9058-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_1854e435-9f58-477d-ac31-53187ee0426d.png?v=1767064613","url":"https:\/\/afsbio.com\/products\/human-tpo-ab-anti-thyroid-peroxidase-antibody-elisa-kit-elk9058","provider":"AFSBio Inc.","version":"1.0","type":"link"}