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Human UBE2L3 (Ubiquitin Conjugating Enzyme E2L3) ELISA Kit

Human UBE2L3 (Ubiquitin Conjugating Enzyme E2L3) ELISA Kit

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Catalogue Number: ELK4425

Alternative Names: L-UBC; E2-F1; UBCH7; UbcM4; Ubiquitin carrier protein L3; Ubiquitin-conjugating enzyme E2-F1; Ubiquitin-protein ligase L3

Species: Human

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research: Enzyme & Kinase;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Conjugating Enzyme E2L3(UBE2L3). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ubiquitin Conjugating Enzyme E2L3(UBE2L3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin Conjugating Enzyme E2L3(UBE2L3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin Conjugating Enzyme E2L3(UBE2L3) in the samples is then determined by comparing the OD of the samples to the standard curve.