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Human UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7) ELISA Kit

Human UGT2B7 (UDP Glucuronosyltransferase 2 Family, Polypeptide B7) ELISA Kit

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Catalogue Number: ELK4673

Alternative Names: UGTB2B9; UDPGTh-2; 3,4-catechol estrogen-specific UDPGT

Species: Human

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research: Enzyme & Kinase;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7(UGT2B7). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to UDP Glucuronosyltransferase 2 Family, Polypeptide B7(UGT2B7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UDP Glucuronosyltransferase 2 Family, Polypeptide B7(UGT2B7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UDP Glucuronosyltransferase 2 Family, Polypeptide B7(UGT2B7) in the samples is then determined by comparing the OD of the samples to the standard curve.