{"product_id":"human-vb1-vitamin-b1-elisa-kit-elk11265","title":"Human VB1 (Vitamin B1) ELISA Kit - ELK11265","description":"\u003cp\u003eHuman VB1 (Vitamin B1) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11265-48, ELK11265-96, ELK11265-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Thiamine; Thiamin; Aneurine\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.63 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 300 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 4.69-300 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Nutrition metabolism; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VB1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200703389875,"sku":"ELK11265-48","price":448.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200703422643,"sku":"ELK11265-96","price":641.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200703455411,"sku":"ELK11265-5x96","price":2725.8,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_aa073157-cc3e-4e5e-ade4-0c1a5a4cb7b4.png?v=1767061877","url":"https:\/\/afsbio.com\/products\/human-vb1-vitamin-b1-elisa-kit-elk11265","provider":"AFSBio Inc.","version":"1.0","type":"link"}