{"product_id":"human-vb6-vitamin-b6-elisa-kit-elk11228","title":"Human VB6 (Vitamin B6) ELISA Kit - ELK11228","description":"\u003cp\u003eHuman VB6 (Vitamin B6) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11228-48, ELK11228-96, ELK11228-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: Pyridoxine\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 1.41 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 300 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 4.69-300 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Nutrition metabolism; Hormone metabolism;\u003c\/p\u003e \n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VB6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VB6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200701718707,"sku":"ELK11228-48","price":576.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200701751475,"sku":"ELK11228-96","price":823.2,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200701784243,"sku":"ELK11228-5x96","price":3498.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_7d86e33e-58f3-48ab-9276-3b4db76359e5.png?v=1767061852","url":"https:\/\/afsbio.com\/products\/human-vb6-vitamin-b6-elisa-kit-elk11228","provider":"AFSBio Inc.","version":"1.0","type":"link"}