{"product_id":"human-vd-vitamin-d-elisa-kit-elk11264","title":"Human VD (Vitamin D) ELISA Kit - ELK11264","description":"\u003cp\u003eHuman VD (Vitamin D) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 48T, 96T, 96T*5\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: ELK11264-48, ELK11264-96, ELK11264-5x96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAlternative Names: VD; VD1; Lamisterol; Vitamin D; Vitamin D1; Lumisterol\u003c\/p\u003e\n\n\u003cp\u003eAssay Type: Competitive Inhibition\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 2.19 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eStandard: 400 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 6.25-400 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eSample Type: Serum, plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eAssay Length: 2h\u003c\/p\u003e\n\n\u003cp\u003eResearch Areas: Metabonomics\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human VD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VD in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eResearch Use Only\u003c\/p\u003e","brand":"ELK Bio","offers":[{"title":"48T","offer_id":45200703226035,"sku":"ELK11264-48","price":371.0,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":45200703258803,"sku":"ELK11264-96","price":526.4,"currency_code":"CAD","in_stock":true},{"title":"96T*5","offer_id":45200703291571,"sku":"ELK11264-5x96","price":2237.2,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/files\/download_360x_23820bba-b4aa-4bdc-85a2-c47c4eb4ee31.png?v=1767061875","url":"https:\/\/afsbio.com\/products\/human-vd-vitamin-d-elisa-kit-elk11264","provider":"AFSBio Inc.","version":"1.0","type":"link"}