{"product_id":"human-vf-visfatin-elisa-kit-e-el-h1763","title":"Human VF (Visfatin) ELISA Kit - E-EL-H1763","description":"\u003cp\u003eHuman VF (Visfatin) ELISA Kit\u003c\/p\u003e\n\n\u003cp\u003eSizes: 24T, 48T, 96T\u003c\/p\u003e\n\n\u003cp\u003eCatalogue Numbers: E-EL-H1763-24, E-EL-H1763-48, E-EL-H1763-96\u003c\/p\u003e\n\n\u003cp\u003eCitations, Manuals and MSDS Available upon request.\u003c\/p\u003e\n\n\u003cp\u003eAbbreviation: VF\u003c\/p\u003e\n\n\u003cp\u003eUNIProt ID: P43490\u003c\/p\u003e\n\n\u003cp\u003eTarget Synonyms: Nampt; NAmPRTase; PBEF1; pre-B-cell colony-enhancing factor 1; Nicotinamide Phosphoribosyltransferase;\u003c\/p\u003e \n\n\u003cp\u003eResearch Areas: Cancer, Cardiovascular, Metabolism, Immunology, Neuroscience, Signal transduction\u003c\/p\u003e\n\n\u003cp\u003eTarget Species: Human\u003c\/p\u003e\n\n\u003cp\u003eApplication: ELISA\u003c\/p\u003e\n\n\u003cp\u003eUsage: This ELISA kit applies to the in vitro quantitative determination of Human VF concentrations in serum, plasma and other biological fluids.\u003c\/p\u003e\n\n\u003cp\u003eTested Sample Types: Serum, Plasma and other biological fluids\u003c\/p\u003e\n\n\u003cp\u003eType: Sandwich\u003c\/p\u003e\n\n\u003cp\u003eAssay Time: 3.5h\u003c\/p\u003e\n\n\u003cp\u003eDetection Type: Colormetric\u003c\/p\u003e\n\n\u003cp\u003eSensitivity: 0.19 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eDetection Range: 0.31-20 ng\/mL\u003c\/p\u003e\n\n\u003cp\u003eTest Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human VF. You can calculate the concentration of Human VF in the samples by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\n\n\u003cp\u003eCross Reactivity: This kit recognizes Human VF in samples. No significant cross-reactivity or interference between Human VF and analogues was observed\u003c\/p\u003e\n\n\u003cp\u003eRecovery Rate: 80%-120%\u003c\/p\u003e\n\n\u003cp\u003ePrecision: Both intra-CV and inter-CV are \u0026lt; 10%.\u003c\/p\u003e\n\n\u003cp\u003eStorage: 2-8℃\/-20℃, 12 months\u003c\/p\u003e\n\n\u003cp\u003eShipping: ice bag\u003c\/p\u003e","brand":"Elabscience","offers":[{"title":"24T","offer_id":43010633302195,"sku":"E-EL-H1763-24","price":210.0,"currency_code":"CAD","in_stock":true},{"title":"48T","offer_id":43010633334963,"sku":"E-EL-H1763-48","price":681.8,"currency_code":"CAD","in_stock":true},{"title":"96T","offer_id":43010633367731,"sku":"E-EL-H1763-96","price":852.6,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0305\/9482\/6376\/products\/Untitled-1_ce1a8718-3c6c-4581-8185-0d122ade566e.jpg?v=1706618119","url":"https:\/\/afsbio.com\/products\/human-vf-visfatin-elisa-kit-e-el-h1763","provider":"AFSBio Inc.","version":"1.0","type":"link"}