Catalogue Number: ELK4487
Alternative Names: WAP1; Putative protease inhibitor WAP1; p53-responsive gene 5 protein
Assay Type: Sandwich
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research: Signal transduction;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WAP Four Disulfide Core Domain Protein 5(WFDC5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to WAP Four Disulfide Core Domain Protein 5(WFDC5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WAP Four Disulfide Core Domain Protein 5(WFDC5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WAP Four Disulfide Core Domain Protein 5(WFDC5) in the samples is then determined by comparing the OD of the samples to the standard curve.